Localization of Ribonucleic Acid in T H E Cytoplasm of Liver Cells*
نویسنده
چکیده
The structure of nucleic acid derived from thymus on the one hand and that derived from yeast on the other were finally established when it was shown by Levene and Jacobs (1) that the former was characterized by a pentose, dextroribose, whereas the other contained (Levene and London, 2) a reduced sugar, desoxyribose. A color reaction described by Feulgen (3) applicable to this sugar has been widely used for the chemical identification of desoxyribonucleic acid and has later been applied (Feulgen and Rossenbeck, 4) with much exactness to the microchemical recognition of this substance in tissues. Numerous observations indicate that the Feulgen reaction is limited to the cell nucleus and our observations are wholly in accord with this conclusion. In 1904 Kohler (3) described a microscope equipped with a fused quartz lens system. The reason for using such a microscope is twofold. One, since the tissue components such as nucleic acid, nucleoproteins, and proteins have absorption bands in the ultraviolet region of the spectrum, unstained sections can be used. Also the resolution is about twice that obtained with visible light. The technique of ultraviolet microscopy has recently been simplified (Lavin, 6) and it is this technique that has been used to obtain the microphotographs shown in this paper. The microphotographs of unstained sections have an astonishing resemblance to photographs of sections stained with nuclear dyes.
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تاریخ انتشار 2003